Synonym: Monoclonal Anti-polyHistidine
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| Related Categories | 9, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, |
| antibody form | ascites fluid |
| clone | HIS-1, monoclonal |
| form | (Suppled as ascites fluid with 15mM sodium azide.) |
| application(s) | dot blot: suitable |
| immunoprecipitation: suitable | |
| indirect ELISA: suitable | |
| western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
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Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry.
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recombinant polyHistidine tagged fusion protein.
Recommended dilution for immunoblotting is 1:3000
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.
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