|Related Categories||9, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags,|
|antibody form||ascites fluid|
|form||(Suppled as ascites fluid with 15mM sodium azide.)|
|application(s)||dot blot: suitable|
|indirect ELISA: suitable|
|western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein|
|shipped in||dry ice|
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Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry.
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recombinant polyHistidine tagged fusion protein.
Recommended dilution for immunoblotting is 1:3000
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Overexpression of phospholemman alters contractility and [Ca2+]i transients in adult rat myocytes Song,J et al. J. Physiol. 283(2), H576-H583, (2002)
Characterization of the six glycosyltransferases involved in the biosynthesis of Yersinia enterocolitica serotype O:3 lipopolysaccharide outer core. Pinta, E., et al. J. Biol. Chem. 285, 28333-42, (2010)
Translocation of Borrelia burgdorferi surface lipoprotein OspA through the outer membrane requires an unfolded conformation and can initiate at the C-terminus. Schulze, R.J., et al. Mol. Microbiol. 76, 1266-78, (2010)
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