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H1506 Sigma

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Synonym: 3α-Hydroxysteroid:NAD(P)+ oxidoreductase

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,
purified by   chromatography
composition   Protein, ≥25%
foreign activity   β-hydroxysteroid dehydrogenase ≤0.5%
  alcohol dehydrogenase ≤0.05%
storage temp.   −20°C

Description

Packaging

50 units in glass bottle

Unit Definition

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

Physical form

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

To standardize a procedure for the enzymatic determination of 3α-Hydroxysteroid dehydrogenase.
Keywords: Chromatography, Enzymology, Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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