• USA Home
  • H1650 - Monoclonal Anti-HLA Class I Antigen antibody produced in mouse

H1650 Sigma

Monoclonal Anti-HLA Class I Antigen antibody produced in mouse

clone W6/32, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-HLA Class I Antigen


Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human, monkey
application(s)   flow cytometry: 5 μL using 1 × 106 cells
  immunoprecipitation: suitable
  indirect immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.
clone   W6/32, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)
biological source   mouse
conjugate   unconjugated



human tonsil leukocyte membrane preparation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide


Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.
4th Workshop: code no. P1
5th Workshop: code nos. BP166, BP288, BP407, B 005

Price and Availability

Antibody Explorer

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?