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H1775 Sigma

Monoclonal Anti-Heat Shock Protein 90 antibody produced in mouse

clone AC-16, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Chaperonins and Heat Shock Proteins,
species reactivity   rabbit, mouse, chicken, wheat germ, Sf9 cell line, human, rat, Achlya
application(s)   microarray: suitable
  western blot: 1:1,000 using cultured human foreskin fibroblasts
clone   AC-16, monoclonal
antibody form   ascites fluid
isotype   IgG2b
mol wt   antigen mol wt 88 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HSP90AA1(3320), HSP90AB1(3326)
mouse ... Hsp90aa1(15519), Hsp90ab1(15516)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

heat shock protein 90 (HSP90) from the water mold Achlya ambisexualis

Specificity

The antibody is reactive with both the constitutive and the inducible forms of HSP90. However, it does not bind to the native form of HSP90. It does not recognize HSP90 from E. coli and yeast.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

esiRNA Knockdown Efficiency Tested by Western Blotting

A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot

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Peer-Reviewed Papers
15

References

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