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H2153 Sigma

HABA/Avidin Reagent

lyophilized powder

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Properties

Related Categories Antibodies, Avidin Reagents, Biotin/Avidin, Supplementary Products
form   lyophilized powder
usage   sufficient for ≤10 assays
color   orange
suitability   suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples
storage temp.   2-8°C

Description

Reconstitution

Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.

Application

HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.

Reagent is capable of detecting biotin at μMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.

General description

4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.

HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.

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Detect Your Unseen Protein
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

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