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  • H3159 - Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

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H3159 Sigma

Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Epigenetics,
species reactivity   mouse, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections
  immunoprecipitation: 50 μg using whole lysate of NIH3T3 cells
  microarray: suitable
  western blot: 1:20,000 using nuclear extract from human HeLa cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HDAC2(3066)
mouse ... Hdac2(15182)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).

General description

Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Physical form

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.

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