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H3274 Sigma

Hygromycin B from Streptomyces hygroscopicus

powder, BioReagent, suitable for cell culture, suitable for insect cell culture

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Properties

Related Categories Aminoglycosides, Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics G-M,
InChI Key   GRRNUXAQVGOGFE-XKIAHZFYSA-N
product line   BioReagent
assay   ≥60% (HPLC)
form   powder
shelf life   4 yr
mol wt   mol wt 527.52 g/mol
application(s)   cell culture | insect: suitable
    (1) 7.1, (2) 8.8
mp   160-180 °C
solubility   H2O: soluble50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.)
suitability   suitable for cell culture
  suitable for insect cell culture
Mode of action   protein synthesis | interferes
storage temp.   2-8°C

Description

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Caution

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

Preparation Note

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Application

Hygromycin B antibiotic was used for hygro resistance effect in the following cells:
• transfected 293T cells with mixture of plasmids at 160 μg/ml.
• MCF-7ALOX12 cells at 150 μg/ml.
• MSCV Luciferase PGK-hygro plasmid.
• STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS.

General description

Aminoglycosides are the most widely used class of antibiotics that target ribosome. Hygromycin B is an aminocyclitol antibiotic. The organism, S. hygroscopicus contains phosphotransferase (HPH) activity, which phosphorylates hygromycin B helping in autoimmunity against the toxic effects of the drug.

Protocols & Applications

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN3462 - class 6.1 - PG 1 - Toxins, extracted from living sources, solid, n.o.s., HI: all (not BR)
WGK Germany 
3
RTECS 
WK2130000
Protocols & Articles

Articles

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Aminoglycosides are a well-known class of antibiotics that are routinely monitored in animal-derived foods. Many countries have instituted regulatory limits for aminoglycosides. There is growing conc...
Emily Barrey, Olga Shimelis, K. G. Espenschied
Reporter US, Volume 32.2
Keywords: Antibiotics, High performance liquid chromatography, Mass spectrometry, Separation, Size-exclusion chromatography, Solid phase extractions

Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere wi...
BioFiles 2006, 1.4, 17.
Keywords: Antibiotics, Environmental, Peptide synthesis

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Agriculture | Crop Protection Workflow

Pesticides are an essential component in modern agriculture and enable growers to fully realize the yield potential of their seeds. From compound discovery to active ingredient development, our chemi...
Keywords: Agriculture, Pesticides

Mouse Embryonic Stem Cell Culture Procedures & Protocols

The development of transgenic and gene knockout technology has provided an effective tool for the analysis of gene function. Critical to this has been the ability to isolate and culture murine embryo...
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Peer-Reviewed Papers
15

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