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  • H3284 - Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit

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H3284 Sigma

Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Gene Regulation,
species reactivity   mouse, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
  immunoprecipitation: 5-10 μL using whole lysate of NIH3T3 cells
  microarray: suitable
  western blot: 1:20,000 using nuclear extract from HeLa human epithelioid carcinoma cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 65 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HDAC1(3065)
mouse ... Hdac1(433759)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 466-482 of human and mouse HDAC1.

General description

Acetylation is a histone modification in which epsilon amino group of conserve lysine residue is modified with enzymes known as histone acetyltransferases (HATs). HATs are present in two forms nuclear and cytoplasmic. Histone deacetylases are competating enzymes of HATs and are often associated with transcriptional repression and nucleosome condensation. Anti-Histone Deacetylase 1 (HDAC1) antibody can be used in chromatin immunoprecipitation. . This antibody can also be used in double immunofloresence labeling. Anti-Histone Deacetylase 1 (HDAC1) antibody specifically recognizes histone deacetylase 1and reacts specifically with HDAC1 of human, rat and mouse.

Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Physical form

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Histone Deacetylase 1 specifically recognizes histone deacetylase 1 by immunoblotting and immunoprecipitation (65 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC1 by immunoblotting is specifically inhibited with the immunizing peptide. The antibody is also useful for the detection of HDAC1 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding, unless there is a protease digestion. The antibody reacts with HDAC1 of human, rat and mouse origin.

Application

Anti-Histone Deacetylase 1 (HDAC1) antibody can be used in immunoblotting; immunoprecipitation and immunohistochemistry. It is also useful in chromatin immunoprecipitation.

Anti-Histone Deacetylase 1 is suitable for immunoblotting; immunoprecipitation and immunohistochemistry. (IHC)
Immunoblotting: a minimum working dilution of 1:20,000 is determined using a nuclear extract of HeLa human epithelioid carcinoma cell line.
Immunoblotting: a minimum working dilution of 1:2,000 is determined using a whole extract of PC-12 rat pheochromocytoma cell line.
Immunoprecipitation: a recommended working volume of 5-10 ml is determined using a whole lysate of NIH 3T3 cells.
Indirect immunoperoxidase staining: a minimum working dilution of 1:500 is determined using protease-digested, formalin-fixed, paraffin-embedded human lymph node sections.

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