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H3375 Sigma

HEPES

≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Properties

Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
Quality Level   ELITE
assay   ≥99.5% (titration)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
Featured Industry   Diagnostic Assay Manufacturing

Description

Frequently Asked Questions

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Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Go to Redi-Dri Product Listing RDD002

Protocols & Applications

Ensure correct concentrations of buffer solutions with the Buffer Calculator

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HEPES buffer solution

1 M in H2O

HEPES sodium salt

≥99.5% (titration)

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Safety & Documentation

Safety Information

WGK Germany 
1
RTECS 
TL6809000

Protocols & Articles

Related Content

Buffer Reference Center

Citric Acid – Na2HPO4 Buffer Solutions, pH approx. 2.6–7.61 Citric acid monohydrate, C6H8O7 • H2O, M. wt. 210.14; 0.1M-solution contains 21.01 g/l. Na2HPO4, M. wt. 141.98; 0.2M-solution contains 28.4...
Keywords: Biological Buffers, Buffers

Peer-Reviewed Papers

References

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Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets. Johan Olerud et al Diabetes 57, 1870-7, (2008)

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Conformational altered p53 as an early marker of oxidative stress in Alzheimer's disease. Buizza L, Cenini G, Lanni C, et al. PLoS ONE 7(1), e29789, (2012)

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Effects of serum-free storage on morphology, phenotype, and viability of ex vivo cultured human conjunctival epithelium. Eidet JR, Utheim OA, Raeder S, et al. Exp. Eye Res. 94(1), 109-16, (2012)

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Direct biomolecule binding on nonfouling surfaces via newly discovered supramolecular self-assembly of lysozyme under physiological conditions. Yang P Macromol. Biosci. 12(8), 1053-9, (2012)

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[Permeability of pannexin 1 channels to large anions]. Romanov RA, Rogachevskaia OA, Kolesnikova AS, et al. Ross. Fiziol. Zh. Im. I. M. Sechenova 98(12), 1578-86, (2012)

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Lipoic acid suppresses compound 48/80-induced anaphylaxis-like reaction. Yun Ho Choi et al Anat. Cell Biol. 43, 317-24, (2010)

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Magnesium alloys: predicting in vivo corrosion with in vitro immersion testing. Walker J, Shadanbaz S, Kirkland NT, et al. J. Biomed. Mater. Res. B. Appl. Biomater. 100(4), 1134-41, (2012)

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Salmonella bongori provides insights into the evolution of the Salmonellae. Fookes M, Schroeder GN, Langridge GC, et al. PLoS Pathog. 7(8), e1002191, (2011)

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Autoantibodies to myelin basic protein (MBP) in healthy individuals and in patients with multiple sclerosis: a role in regulating cytokine responses to MBP. Chris J Hedegaard et al Immunology 128, e451-61, (2009)

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Ratiometric fluorescence sensor based on a pyrene derivative and quantification detection of heparin in aqueous solution and serum. Dai Q, Liu W, Zhuang X, et al. Anal. Chem. 83(17), 6559-64, (2011)

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Ceramide-1-phosphate, in contrast to ceramide, is not segregated into lateral lipid domains in phosphatidylcholine bilayers. Morrow MR Biophys. J. 96(6), 2216-26, (2009)

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A novel selective muscarinic acetylcholine receptor subtype 1 antagonist reduces seizures without impairing hippocampus-dependent learning. Sheffler DJ, Williams R, Bridges TM, et al. Mol. Pharmacol. 76(2), 356-68, (2009)

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Pyrophosphate sensing by a fluorescent Zn2+ bound triazole linked imino-thiophenyl conjugate of calix[4]arene in HEPES buffer medium: spectroscopy, microscopy, and cellular studies. Pathak RK, Tabbasum K, Rai A, et al. Anal. Chem. 84(11), 5117-23, (2012)

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Myosin heavy chain isoform composition and Ca(2+) transients in fibres from enzymatically dissociated murine soleus and extensor digitorum longus muscles. Calderón JC J. Physiol. 588(Pt 1), 267-79, (2010)

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Low plasma membrane expression of the miltefosine transport complex renders Leishmania braziliensis refractory to the drug. María P. Sánchez-Cañete et al Antimicrob. Agents Chemother. 53, 1305-13, (2009)

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Synthesis and bio-imaging application of highly luminescent mercaptosuccinic acid-coated CdTe nanocrystals. Ying E PLoS ONE 3(5), e2222, (2008)

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A fluorescence "turn-on" chemodosimeter for Cu2+ in aqueous solution based on the ion promoted oxidation. Li J, Zeng Y, Hu Q, et al. Dalton Trans. 41(13), 3623-6, (2012)

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Whole-exome sequencing of DNA from peripheral blood mononuclear cells (PBMC) and EBV-transformed lymphocytes from the same donor. Londin ER BMC Genomics 12, 464, (2011)

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A Zn2+ specific triazole based calix[4]arene conjugate (L) as a fluorescence sensor for histidine and cysteine in HEPES buffer milieu. Pathak RK, Tabbasum K, Rai A, et al. Analyst 137(17), 4069-75, (2012)

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Mechanism for modulation of gating of connexin26-containing channels by taurine. Locke D, Kieken F, Tao L, et al. J. Gen. Physiol. 138(3), 321-39, (2011)

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Highly selective fluorescence turn-on sensing of gold ions by a nanoparticle generation/C-I bond cleavage sequence. Park J, Choi S, Kim TI, et al. Analyst 137(19), 4411-4, (2012)

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The diamidine DB75 targets the nucleus of Plasmodium falciparum. Purfield AE Malaria Journal 8, 104, (2009)

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Transient acidification and subsequent proinflammatory cytokine stimulation of astrocytes induce distinct activation phenotypes. Renner NA, Sansing HA, Inglis FM, et al. J. Cell Physiol. 228(6), 1284-94, (2013)

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Global response of Plasmodium falciparum to hyperoxia: a combined transcriptomic and proteomic approach. Torrentino-Madamet M Malaria Journal 10, 4, (2011)

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High-resolution structures of Neotermes koshunensis β-glucosidase mutants provide insights into the catalytic mechanism and the synthesis of glucoconjugates. Jeng WY, Wang NC, Lin CT, et al. Acta Crystallogr. D Biol. Crystallogr. 68(Pt 7), 829-38, (2012)

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Killer Ig-like receptor (KIR) genotype predicts the capacity of human KIR-positive CD56dim NK cells to respond to pathogen-associated signals. Korbel DS J. Immunol. 182(10), 6426-34, (2009)

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pH-dependent inhibition of native GABA(A) receptors by HEPES. Hugel S, Kadiri N, Rodeau JL, et al. Br. J. Pharmacol. 166(8), 2402-16, (2012)

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Exercise reduces GABA synaptic input onto nucleus tractus solitarii baroreceptor second-order neurons via NK1 receptor internalization in spontaneously hypertensive rats. Chen CY J. Neurosci. 29(9), 2754-61, (2009)

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Prestorage in ovo injection of biological buffers: an approach to improve hatchability in long-term stored eggs. Akhlaghi A, Jafari Ahangari Y, Hashemi SR, et al. Poult. Sci. 92(4), 874-81, (2013)

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The antidiabetic drug ciglitazone induces high grade bladder cancer cells apoptosis through the up-regulation of TRAIL. Plissonnier ML, Fauconnet S, Bittard H, et al. PLoS ONE 6(12), e28354, (2011)

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A model of a MAPK•substrate complex in an active conformation: a computational and experimental approach. Lee S, Warthaka M, Yan C, et al. PLoS ONE 6(4), e18594, (2011)

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Biological pH buffers in IVF: help or hindrance to success. Will MA, Clark NA, and Swain JE J. Assist. Reprod. Genet. 28(8), 711-24, (2011)

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A novel amiloride-sensitive h+ transport pathway mediates enhanced superoxide production in thick ascending limb of salt-sensitive rats, not na+/h+ exchange. Paul M. O’Connor et al Hypertension 54, 248-54, (2009)

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Characterization of cortical neuronal and glial alterations during culture of organotypic whole brain slices from neonatal and mature mice. Jerome A. Staal et al PLoS ONE 6, e22040, (2011)

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Gravity, a regulation factor in the differentiation of rat bone marrow mesenchymal stem cells. Yan Huang et al J. Biomed. Sci. 16, 87, (2009)

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Tris buffer improves fluorescence yield of ram spermatozoa when evaluating membrane integrity. Yániz JL, Mateos JA, and Santolaria P Microsc. Res. Tech. 75(4), 520-3, (2012)

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Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster. Vognsen T and Kristensen O Biochem. Biophys. Res. Commun. 420(1), 188-92, (2012)

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Role of bicarbonate as a pH buffer and electron sink in microbial dechlorination of chloroethenes. Delgado AG, Parameswaran P, Fajardo-Williams D, et al. Microb. Cell Fact. 11, 128, (2012)

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Hydrothermal synthesis and properties of controlled α-Fe2O3 nanostructures in HEPES solution. Li H, Lu Z, Li Q, et al. Chem. Asian J. 6(9), 2320-31, (2011)

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Phosphate and HEPES buffers potently affect the fibrillation and oligomerization mechanism of Alzheimer's Aβ peptide. Garvey M, Tepper K, Haupt C, et al. Biochem. Biophys. Res. Commun. 409(3), 385-8, (2011)

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HOPE-preservation of paraffin-embedded sputum samples--a new way of bioprofiling in COPD. Pedersen F, Marwitz S, Seehase S, et al. Respir. Med. 107(4), 587-95, (2013)

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What's in your buffer? Solute altered millisecond motions detected by solution NMR. Wong M, Khirich G, and Loria JP Biochemistry 52(37), 6548-58, (2013)

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Magnetosomes on surface: an imaging study approach. Gojzewski H, Makowski M, Hashim A, et al. Scanning 34(3), 159-69, (2012)

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Assessment of cytochrome C oxidase dysfunction in the substantia nigra/ventral tegmental area in schizophrenia. Rice MW, Smith KL, Roberts RC, et al. PLoS ONE 9(6), e100054, (2014)

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Proton feedback mediates the cascade of color-opponent signals onto H3 horizontal cells in goldfish retina. Kamiji NL, Yamamoto K, Hirasawa H, et al. Neurosci. Res. 72(4), 306-15, (2012)

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Estrogen partially down-regulates PTEN to prevent apoptosis in VSC4.1 motoneurons following exposure to IFN-gamma. Smith JA, Zhang R, Varma AK, et al. Brain Res. 1301, 163-70, (2009)

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The tissue is the issue: improved methylome analysis from paraffin-embedded tissues by application of the HOPE technique. Marwitz S, Kolarova J, Reck M, et al. Lab. Invest. 94(8), 927-33, (2014)

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Further electrophysiological studies on cellular effect of herbicide, bromoxynil, using characean cells. Shimmen T J. Plant Res. 125(6), 749-54, (2012)

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High fat diet-induced obesity leads to proinflammatory response associated with higher expression of NOD2 protein. Kim MS, Choi MS, and Han SN Nutr. Res. Pract. 5(3), 219-23, (2011)

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Effect of 5/6 nephrectomized rat serum on epithelial-to-mesenchymal transition in vitro. Lu Z Ren. Fail. 33(6), 600-8, (2011)

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Reactions of buffers in cyanogen bromide-induced ligations. Vogel H, Gerlach C, and Richert C Nucleosides Nucleotides Nucleic Acids 32(1), 17-27, (2013)

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Connexin-based channels in astrocytes: how to study their properties. Giaume C, Orellana JA, Abudara V, et al. Methods Mol. Biol. 814, 283-303, (2012)

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Merck 14,4654

Beil. 23,V,2,379

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