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H3506 Sigma

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

Synonym: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 5 g in poly bottle

100, 500 mg in poly bottle

Unit Definition

One unit will cause a change in A600 of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 ml reaction mixture (45 minute assay).

Application

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.

Price and Availability


Amplified Detection

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/shippingandlong-termstoragev3.Par.0001.File.tmp/shippingandlong-termstoragev3.pdf
How is Hyaluronidase from bovine testes, Product H3506, solubilized?
It is soluble at 1 mg/ml in 0.02 M phosphate buffer, pH 7 containing 77 mM sodium chloride and 0.01% bovine serum albumin.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/sigma/product%20information%20sheet/h3506pis.pdf
How is a solution of Hyaluronidase from bovine testes, Product H3506, stored?
Prepare fresh solutions directly before use since stock solutions are not stable for storage.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/sigma/product%20information%20sheet/h3506pis.pdf
Can Hyaluronidase from bovine testes, Product H3506, be used on humans?
No. None of our packaged research products are for human use. 
What is the shelf life of Product H3506, Hyaluronidase from bovine testes?
Product H3506, Hyaluronidase from bovine testes, has a Recommended Retest period of two years (period of time between the Quality Control Release and Recommended Retest dates).  This date assigns the time frame during which the batch is expected to remain within established specifications if stored under Sigma-Aldrich’s defined conditions, which for product H3506 is at -20°C.  This information is provided on the Product's lot specific Certificate of Analysis.     For more information on our Product Dating Policies, please click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/product-dating-information-statementv4.Par.0001.File.tmp/product-dating-information-statement-v4.pdf.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
Vicki Caligur
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatography, Coagulation, Degradations, Enzyme-linked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, Help, High performance liquid chromatography, Inflammation, Ion-exchange chromatography, Ligands, Mass spectrometry, Methods, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain reaction, Polymerization reactions, Proteomics, Reductive aminations, Sequences, Spectroscopy, Sulfations, Transcription, Type, transformation

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

The turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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H3757 Hyaluronidase from bovine testes, Type VIII, lyophilized powder, 300-1,000 U/mg Sterile filtered prior to lyophilization, recommended for biological fluid analysis

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