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H3524 Sigma

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse

clone LK2, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Chaperonins and Heat Shock Proteins,
species reactivity   E. coli, helminth, spinach, chicken, human, rat
application(s)   electron microscopy: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  indirect ELISA: suitable
  western blot: 1:100 using cultured human foreskin fibroblast extract
clone   LK2, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 60 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HSPD1(3329)
rat ... Hspd1(63868)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human heat shock protein 60 (HSP60).

General description

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. The 60 kDa HSP family (HSP60) which retained a uniquely high level of sequence conservation during evolution is a focus of interest as a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia. In human arthritis and experimentally-induced arthritis in animals, disease development was seen to coincide with development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homologue. A human mitochondrial protein, originally designated P1, has been described as the human homologue of the bacterial HSP60, and >45% of the protein has sequence identity with its bacterial homologue (groEL, HSP65).

Monoclonal Anti-Heat Shock Protein 60 (HSP60), clone LK2, recognizes an epitope located between amino acid residues 383-419 of the human (corresponding to a.a. residues 356-393 of the mycobacterial) HSP60. The antibody is reactive against the mammalian (e.g., human, rat), avian (e.g., chicken), bacterial (e.g., E. coli), helminths and spinach HSP60. In immunoblotting, the antibody may label additional bands at approx. 38 and 97 kDa. It is reactive with both the constitutive and the inducible HSP60. It shows a raised level of staining in immunohistochemistry of formalin-fixed, paraffin-embedded synovial membranes taken from patients with juvenile chronic arthritis.

Application

Studies, using monoclonal anti-mycobacterial HSP60 antibody clone LK2 that cross reacts with human HSP60, have shown increased expression of HSP60 in inflamed tissue. However, because of this cross reactivity, it was unable to distinguish between the expression of HSP60 from bacterial origin (e.g., after a bacterial infection) or endogenous self-HSP60. The availability of monoclonal antibody that is reactive with both the mammalian and bacterial HSP60, together with monoclonal antibody which has a unique specificity for mammalian HSP60 (clone LK1), enable the differentiation between the HSP60 of mammalian and bacterial origin. The product may be used in ELISA, immunoblotting and immunocytochemistry.

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