Synonym: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
|Related Categories||3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,|
|mol wt||mol wt ~55 kDa (four subunits of 14 kDa each)|
|composition||Protein, 80-105% biuret|
|suitability||mouse embryo tested|
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.1,2
These enzymes randomly cleave β-N-acetylhexosamine-[1→4
One unit will cause a change in A600 of 0.330 per minute at pH 5.7 at 37°C in a 2.0 ml reaction mixture (45 minute assay).
Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass from newly ovulated eggs, when preparing the eggs for in vitro culture.
Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.
Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.
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Type I-S, lyophilized powder, 400-1000 units/mg solid
Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
Type VIII, lyophilized powder, 300-1,000 U/mg
Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid
1. OBJECTIVE To standardize a procedure for determining the activity of Hyaluronidase at Sigma-Aldrich St. Louis.
Keywords: Extinction coefficient
Comparison of bovine- and recombinant human-derived hyaluronidase with regard to fertilization rates and embryo morphology in a sibling oocyte model: a prospective, blinded, randomized study. Taylor TH, Elliott T, et. al. Fertil. Steril. 85, 1544-1546, (2006)
Meyer, K., Hoffman, P., Linker, A. The Enzymes 2nd ed.,, (1960) 4, 447
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