Synonym: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
|Related Categories||3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,|
|mol wt||mol wt ~55 kDa (four subunits of 14 kDa each)|
|composition||Protein, 80-105% biuret|
|suitability||mouse embryo tested|
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.1,2
These enzymes randomly cleave β-N-acetylhexosamine-[1→4
One unit will cause a change in A600 of 0.330 per minute at pH 5.7 at 37°C in a 2.0 ml reaction mixture (45 minute assay).
Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass from newly ovulated eggs, when preparing the eggs for in vitro culture.
Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.
Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.
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Type I-S, lyophilized powder, 400-1000 units/mg solid
Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
Type VIII, lyophilized powder, 300-1,000 U/mg
Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid
The turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Comparison of bovine- and recombinant human-derived hyaluronidase with regard to fertilization rates and embryo morphology in a sibling oocyte model: a prospective, blinded, randomized study. Taylor TH, Elliott T, et. al. Fertil. Steril. 85, 1544-1546, (2006)
Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation Spectroscopy. Rich RM, Mummert M, Foldes-Papp Z, et al. J. Photochem. Photobiol. B, Biol. 116, 7-12, (2012)
A randomized clinical trial of recombinant human hyaluronidase-facilitated subcutaneous versus intravenous rehydration in mild to moderately dehydrated children in the emergency department. Spandorfer PR, Mace SE, Okada PJ, et al. Clin. Ther. 34(11), 2232-45, (2012)
Bioactive hyaluronan fragment (hexasaccharide) detects specific hexa-binding proteins in human breast and stomach cancer: possible role in tumorogenesis. Srinivas P, Kollapalli SP, Thomas A, et al. Indian J. Biochem. Biophys. 49(4), 228-35, (2012)
Identification of amino acid residues required for the substrate specificity of human and mouse chondroitin sulfate hydrolase (conventional hyaluronidase-4). Kaneiwa T, Miyazaki A, Kogawa R, et al. J. Biol. Chem. 287(50), 42119-28, (2012)
Hyaluronan (HA) interacting proteins RHAMM and hyaluronidase impact prostate cancer cell behavior and invadopodia formation in 3D HA-based hydrogels. Gurski LA, Xu X, Labrada LN, et al. PLoS ONE 7(11), e50075, (2012)
Murine hyaluronidase 2 deficiency results in extracellular hyaluronan accumulation and severe cardiopulmonary dysfunction. Chowdhury B, Hemming R, Hombach-Klonisch S, et al. J. Biol. Chem. 288(1), 520-8, (2013)
The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition. Redgrove KA, Nixon B, Baker MA, et al. PLoS ONE 7(11), e50851, (2012)
Phlorotannin extracts from fucales characterized by HPLC-DAD-ESI-MSn: approaches to hyaluronidase inhibitory capacity and antioxidant properties. Ferreres F, Lopes G, Gil-Izquierdo A, et al. Mar. Drugs 10(12), 2766-81, (2012)
[The criteria of differentiated diagnostics of early arthritis on the basis of analysis of serum hyaluronidase and deoxyribonuclease activity]. Volkova MV and Kunder EV Klin. Lab. Diagn. (10), 22-6, (2012)
Structural analysis of the phimotic prepuce in patients with failed topical treatment compared with untreated phimosis. Favorito LA, Balassiano CM, Rosado JP, et al. Int. Braz J Urol 38(6), 802-8, (2012)
Hyaluronidase from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): Cloning, structural modeling, purification, and immunological analysis. Justo Jacomini DL, Campos Pereira FD, Aparecido dos Santos Pinto JR, et al. Toxicon 64, 70-80, (2013)
Rapid insulin-mediated increase in microvascular glycocalyx accessibility in skeletal muscle may contribute to insulin-mediated glucose disposal in rats. Eskens BJ, Mooij HL, Cleutjens JP, et al. PLoS ONE 8(1), e55399, (2013)
KIAA1199, a deafness gene of unknown function, is a new hyaluronan binding protein involved in hyaluronan depolymerization. Yoshida H, Nagaoka A, Kusaka-Kikushima A, et al. Proc. Natl. Acad. Sci. U. S. A. 110(14), 5612-7, (2013)
[Optimization of Streptococcus equi subsp. zooepidemicus cultivation process--producer of hyaluronic acid]. Tsepilov RN, Beloded AV, and Samoĭlenko II Zh. Mikrobiol. Epidemiol. Immunobiol. (2), 12-20, (2013)
Meyer, K., Hoffman, P., Linker, A. The Enzymes 2nd ed.,, (1960) 4, 447
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