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H4281 Sigma

EX-CELL® Hybridoma Medium

without antibiotics, antimycotics, L-glutamine, and phenol red, with sodium bicarbonate, HEPES, essential and non-essential amino acids, liquid, sterile-filtered, suitable for hybridoma

Synonym: Hybridoma Medium



Related Categories Cell Culture, Hybridoma Media, Hybridoma Platform, Serum-free Media
Quality Level   GMP
description   endotoxin tested
  for research or for further manufacturing use
sterility   sterile-filtered
form   liquid
suitability   suitable for hybridoma
storage temp.   2-8°C



Serum-free, low-protein medium formulated to support the growth of hybridomas used in the manufacture of monoclonal antibodies.


Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label.

Features and Benefits

Designed to support the rapid growth of cells to high densities and the maintenance of viable cells for extended culture periods. This formulation reduces raw material cost and the low protein level reduces cost associated with downstream processing. Purified components in the product reduce the variability sometimes encountered with biological materials leading to improved consistency of the medium performance. Cells can be successfully transferred from serum-supplemented cultures to this medium with little or no adaptation.

Other Notes

This proprietary formulation includes inorganic salts, essential and non-essential amino acids, vitamins, sodium bicarbonate, HEPES, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and human transferrin (source tested negative for HIV antibody and HbsAG). The medium does not contain L-glutamine, antibiotics, antimycotics, and phenol red.


Prior to use, medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM L-glutamine.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability

All labs need water
Protocols & Articles
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