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H4502 Sigma

Hexokinase from Saccharomyces cerevisiae

Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

Synonym: ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
type   Type F-300
form   lyophilized powder
purified by   crystallization
anion traces   sulfate (SO42-): free
foreign activity   ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
storage temp.   −20°C
Gene Information   bakers yeast ... HXK1(850614), HXK2(852639)

Description

Packaging

2500, 10000 units in poly bottle

500, 1000 units in glass bottle

Quality

A mixture of isoenzymes.

Reconstitution

Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.

Unit Definition

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing approx. 15% sodium citrate

Biochem/physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of Hexokinase

This procedure applies to most products1 that have a specification for Hexokinase determination by enzymatic determination.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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