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H4522 Sigma

Human Serum

from human male AB plasma, USA origin, sterile-filtered

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Properties

Related Categories Cell Culture, Fetal Bovine Serum (FBS) and Other Animal Sera, Human Sera More...
biological source   from human male AB plasma
sterility   sterile-filtered
composition   hemoglobin, ≤20 mg/dL
origin   USA origin
impurities   ≤10 EU/mL endotoxin
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Endotoxin tested

Warning

Each donor is tested for and found non-reactive for Hepatitis B & C and non-reactive for Human Immunodeficiency Virus (HIV) antibody by ELISA. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly.

Application

Human AB serum is used in tissue engineering, transplantation and cell therapy applications for the expansion of mesenchymal stem cells (MSC) from adipose tissue or mesenchymal stromal cells from human bone marrow; for standardized limbal epithelial stem cell graft generation and transplantation; for ex vivo expansion of NK cells from peripheral blood in Hematopoeitic Stem Cell Expansion Medium; and for upgrading pretransplant human islet culture technology.

Price and Availability


Cell Freezing

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
There is a precipitate in my serum.  Is my serum contaminated?
Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Is Product H4522, Human serum, Heat Inactivated (HI)?
This serum is not heat inactivated. If heat inactivated serum is needed, the serum can be heated to 56 °C for 30 minutes. Heating inactivates the complement cascade. Active complement can participate in cytolytic events, leading to contraction of smooth muscle, histamine release from mast cells and platelets, and activation of lymphocytes and macrophages.
How is Product H4522, Human serum, prepared?
Blood is collected and has citrate added as an anticoagulant to inhibit clotting.  The blood is then centrifuged and separated into cells and plasma.  The plasma is separated from the cells, and calcium is added to the plasma to activate the clotting cascade.  The preparation is then centrifuged again, and the serum is the liquid product remaining.  This process yields a serum low in growth factors, as it does not contain those factors released from the cells upon clotting.
Product H4522, Human serum, is cloudy. What causes the cloudiness?
It is not unusual for our human serum products to be cloudy. This product is from pooled serum and cloudiness indicates that lipids are present in the serum. No clear, lipid-free human serum products are available.
Why do we specify male AB in the description of Product H4522, Human serum?
Male blood is used in serum products, where specified, because female serum can have more antibodies resulting from pregnancy.  Type AB serum is used as there are no anti-A and/or anti-B antibodies that are found in other blood types.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the difference between human serum from AB plasma and  human serum from AB clotted whole blood?
Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required. Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum. Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present.  The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914. We offer both products heat inactivated. Product H5667 Human Serum Heat Inactivated, from male AB clotted whole blood Product H3667 Human Serum Heat Inactivated, from human male AB plasma
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles
Peer-Reviewed Papers
15

References

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