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H4652 Sigma

HMG-1 human

lyophilized powder, ≥90% (SDS-PAGE), Histidine-tagged, recombinant, expressed in E. coli

Synonym: Amphoterin, HMGB1, High-Mobility-Group protein

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Gene Regulation and Expression, Transcription Factors
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   lyophilized powder
mol wt   mol wt 25 kDa (215 amino acids; comprises two homologous HMG boxes, rich in basic amino acids, and an acidic tail at the carboxy-terminus)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HMGB1(3146)

Description

Analysis Note

Tested in DNA retardation assay

Physical form

Lyophilized powder containing HEPES buffer salts, pH 7.8, NaCl, EDTA, Triton X-100, Leupeptin and AEBSF.

Application

HMG-1 (high-mobility-group protein) human has been used:
• to prepare a standard curve for measuring HMG1 using ELISA (enzyme-linked immunosorbent assay) test
• to study the apoptotic ratios of Lewis cells in presence of exogenous HMG-1
• to study the changes in Th (T helper)-17 cell expansion in the presence of HMG-1 under in vitro conditions
• for the migration assay of GM7373 cells

Biochem/physiol Actions

HMG-1 (high mobility group protein) is a non-histone chromosomal protein, which promotes the sequence specific DNA attachment of proteins. HMG1 binds DNA in a non-sequence specific manner. It is involved in DNA replication, transcription and repair. In addition, it is up-regulated in cancers and is associated with cellular growth, vasculogenesis and apoptosis regulation. In the presence of infection or injury, HMG1 is secreted and mediates inflammation. It is associated with the pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE).

General description

HMG-1 (high mobility group protein) is a nuclear protein of molecular weight 30kDa. It is secreted from macrophages, monocytes and necrotic cells. It is ubiquitously expressed. The protein has basic HMG (high mobility group) box domains and an acidic carboxyl-terminus.

Price and Availability


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Safety & Documentation

Safety Information

Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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