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H4772 Sigma

Monoclonal Anti-Hamster Immunoglobulins (IgG and IgM) antibody produced in mouse

clone HG-31, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, H1-HE, Hamster Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   mouse
antibody form   ascites fluid
clone   HG-31, monoclonal
mol wt   antigen mol wt 150-200 kDa
contains   15 mM sodium azide
does not react with   human, canine (IgG), rabbit (IgG), bovine (IgG), sheep (IgG), chicken (IgG), guinea pig (IgG), goat (IgG), pig (IgG), cat (IgG), horse (IgG), rat (IgG)
application(s)   capture ELISA: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  indirect ELISA: 1:10,000
  western blot: suitable using denatured, non-reduced hamster serum
isotype   IgG2a
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified hamster IgG

Application

Monoclonal Anti-Hamster Immunoglobulins (IgG and IgM) antibodies are suitable for use in flow cytometry . These antibodies can also be used for ELISA (1:10,000), dot blot, immunocytochemistry and western blot.

General description

Primary hamster antibodies are often used for various research and clinical assays. Thus, secondary anti-hamster antibodies are useful tools for the detection and localization of target proteins that are bound to primary hamster antibodies. As secondary anti-hamster antibodies may lack specificity, their use may result in generation of false positive results and increased background staining. In such instances, the use of monoclonal anti-hamster immunoglobulins can enhance the accuracy of test results as they do not bind to non-related antibodies present in the test sample
Monoclonal Anti-Hamster Immunoglobulins are specific for an epitope located on the heavy chain of hamster IgG and IgM. The immunoglobulins do not react with serum or IgGs from humans. Additionally, the antibodies do not cross-react with IgGs from guinea pig, rat, bovine, rabbit, goat, sheep, horse, dog, chicken, pig or cat.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

WGK Germany 
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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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