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H4777 Sigma

Heparan sulfate proteoglycan

≥400 μg/mL glycosaminoglycan, 0.2 μm filtered

Synonym: HSPG

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Description

Components

Heparan sulfate proteoglycan is a large biomolecule composed of a core protein covalently bound to heparin sulfate chains that is an integral part of the basement membrane. The number of polysaccharide chains and the size of the core protein are variable according to the source.

Application

Heparan Sulfate promotes the attachment of cells in culture and induces high affinity binding of FGF-basic to cells deficient in heparin sulfate and soluble FGF receptors at a concentration of 10-100 ng/mL.

Biochem/physiol Actions

This is a multifunctional molecule. As a component of the extracellular matrix that binds to fibroblast growth factors, vascular endothelial growth factor and vascular endothelial growth factor receptors. It also acts as a docking molecule for matrilysin and other matrix metalloproteinases.

Caution

It is recommended to store this product at 20°C, where it will retain its function for 3 years

Preparation Note

This product is isolated from the basement membrane of Engelbreth-Holm-Swarm mouse sarcoma in a solution containing 50 mM Tris HCl at pH 7.4, 150 mM NaCl, 1 mM EDTA, and 0.1 mM PMSF.

Price and Availability


Amplified Detection

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
Vicki Caligur
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatography, Coagulation, Degradations, Enzyme-linked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, Help, High performance liquid chromatography, Inflammation, Ion-exchange chromatography, Ligands, Mass spectrometry, Methods, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain reaction, Polymerization reactions, Proteomics, Reductive aminations, Sequences, Spectroscopy, Sulfations, Transcription, Type, transformation

Glycosaminoglycans and Proteoglycans

Glycosaminoglycans (GAGs) are large linear polysaccharides constructed of repeating disaccharide units with the primary configurations containing an amino sugar (either GlcNAc or GalNAc) and an uroni...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Catalysis, Coagulation, Degradations, Growth factors, Inflammation, Ligands, Pentose phosphate pathway, Polymerization reactions, Sulfations

Peer-Reviewed Papers
15

References

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