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H4890 Sigma

Anti-Human Hemoglobin antibody produced in rabbit

whole antiserum

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Human Plasma Proteins, H1-HE, Primary Antibodies,
species reactivity   human
application(s)   indirect ELISA: 1:8,000
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HBB(3043)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Hemoglobin isolated from normal adult human red blood cells and treated to remove lipoproteins.

Preparation Note

treated to remove lipoproteins

Application

Anti-Human Hemoglobin antibody produced in rabbit was used for immunoblotting using crude human cerebellar protein extracts and membrane extracts of human RBC. It was used for immunohistochemistry of human tongue cancer samples.

Biochem/physiol Actions

Hemoglobin (Hb) is oxygen-binding metalloprotein that is present in the red blood cells. The most abundant Hb is HbA tetramer that is made up of two α, two β chains and the nonprotein heme group. The heme group contains iron ion and a porphyrin ring. Mutations in Hb gene result in hemoglobinopathies, the best known being sickle-cell disease and thalassemias.

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