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H4891 Sigma

Hanks’ Balanced Salts

Modified, without calcium chloride, magnesium sulfate, phenol red and sodium bicarbonate, powder, suitable for cell culture

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Properties

Related Categories Balanced Salt Mixtures and Solutions, Cell Culture, Classic Media and Salts, Hank’s Balanced Salts
Quality Level   GMP
form   powder
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Other Notes

The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Application

Hanks′ Balanced Salts may be used when working with stem cells or when growing cells at low densities. Also, it may be used to minimize cell attachment or aggregation, and for the preparation of external bathing solution and wash solution.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Peer-Reviewed Papers
15

References

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M2643 Magnesium sulfate, BioReagent, suitable for cell culture, suitable for insect cell culture
C5670 Calcium chloride, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
P3532 Phenol Red, powder, BioReagent, suitable for cell culture
S5761 Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

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