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H5527 Sigma

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H2O, γ-irradiated

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Properties

Related Categories Aminoglycosides, Antibiotics, Antibiotics A to Z, Antibiotics G-M, Antibiotics by Application,
biological source   from Streptomyces hygroscopicus
InChI Key   GRRNUXAQVGOGFE-XKIAHZFYSA-N
sterility   γ-irradiated
assay   ≥60% (HPLC)
concentration   45-60 mg/mL in H2O
color   yellow-brown
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   viruses
storage temp.   2-8°C

Description

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Biochem/physiol Actions

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Caution

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Preparation Note

This product is a solution that is =60% (HPAE), 45-60 mg/mL in H2O, and γ-irradiated .

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN2810 - class 6.1 - PG 1 - PAX forbidden - Toxic, liquids, organic, n.o.s., HI: all
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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