|Related Categories||Aminoglycosides, Antibiotics, Antibiotics A to Z, Antibiotics G-M, Antibiotics by Application,|
|concentration||45-60 mg/mL in H2O|
Hygromycin has been used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications.
Hygromycin B is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. It is used in gene transfer experiments as a selection agent. Product H5527 is a solution that is ≥60% (HPAE), 45-60 mg/mL in H2O, and γ-irradiated .
Antibacterial and antifungal. Mode of action: Inhibition of protein synthesis, by inducing the misreading of the m-RNA template in prokaryotes and eukaryotes. It selectively penetrates cells that have been rendered permeable by virus infection.
Customers Also Viewed
50 mg/mL in H2O, sterile-filtered, BioReagent, suitable for cell culture
powder, BioReagent, suitable for cell culture, suitable for insect cell culture
plant cell culture tested, BioReagent, ≥60% (HPAE), lyophilized powder
≥60% (HPAE), 45-60 mg/mL in H2O
Certificate of Analysis
Certificate of Origin
|Symbol||GHS05, GHS06, GHS08|
|Hazard statements||H301 + H311-H318-H330-H334|
|Precautionary statements||P260-P280-P284-P301 + P310-P305 + P351 + P338-P310|
|Personal Protective Equipment||Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter|
|Hazard Codes (Europe)||T+|
|Risk Statements (Europe)||26/27/28-41-42/43|
|Safety Statements (Europe)||23-26-28-36/37/39-45|
|RIDADR||UN 2810 6.1/PG 1|
Accumulation of the FACT complex, as well as histone H3.3, serves as a target marker for somatic hypermutation. Aida M, Hamad N, Stanlie A, et al. Proc. Natl. Acad. Sci. U. S. A. 110(19), 7784-9, (2013)
Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker. Gruber S, Omann M, Rodrìguez CE, et al. BMC Res. Notes 5, 641, (2012)
Plant regeneration and genetic transformation of C. canadensis: a non-model plant appropriate for investigation of flower development in Cornus (Cornaceae). Liu X, Feng CM, Franks R, et al. Plant Cell Rep. 32(1), 77-87, (2013)
A system for the measurement of gene targeting efficiency in human cell lines using an antibiotic resistance-GFP fusion gene. Konishi Y, Karnan S, Takahashi M, et al. Biotechniques 53(3), 141-52, (2012)
Spontaneous transmitter release recruits postsynaptic mechanisms of long-term and intermediate-term facilitation in Aplysia. Jin I, Udo H, Rayman JB, et al. Proc. Natl. Acad. Sci. U. S. A. 109(23), 9137-42, (2012)
Spontaneous transmitter release is critical for the induction of long-term and intermediate-term facilitation in Aplysia. Jin I, Puthanveettil S, Udo H, et al. Proc. Natl. Acad. Sci. U. S. A. 109(23), 9131-6, (2012)
Agrobacterium tumefaciens-mediated transformation for investigating pathogenicity genes of the phytopathogenic fungus Colletotrichum sansevieriae. Nakamura M, Kuwahara H, Onoyama K, et al. Curr. Microbiol. 65(2), 176-82, (2012)
Expression of wheat Na(+)/H(+) antiporter TNHXS1 and H(+)- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance. Gouiaa S, Khoudi H, Leidi EO, et al. Plant Mol. Biol. 79(1-2), 137-55, (2012)
Efficient Agrobacterium-mediated transformation of the liverwort Marchantia polymorpha using regenerating thalli. Kubota A, Ishizaki K, Hosaka M, et al. Biosci. Biotechnol. Biochem. 77(1), 167-72, (2013)
Chiral pool based efficient synthesis of the aminocyclitol core and furanoside of (-)-hygromycin A: formal total synthesis of (-)-hygromycin A. Lo HJ, Chang YK, and Yan TH Org. Lett. 14(23), 5896-9, (2012)
Detection of probable marker-free transgene-positive rice plants resistant to rice tungro disease from backcross progenies of transgenic Pusa Basmati 1. Roy S, Banerjee A, Tarafdar J, et al. J. Genet. 91(2), 213-8, (2012)
Capreomycin and hygromycin B modulate the catalytic activity of the delta ribozyme in a manner that depends on the protonation and complexation with Cu2+ ions of these antibiotics. Szafraniec M, Stokowa-Sołtys K, Nagaj J, et al. Dalton Trans. 41(32), 9728-36, (2012)
Generation of astaxanthin mutants in Xanthophyllomyces dendrorhous using a double recombination method based on hygromycin resistance. Niklitschek M, Baeza M, Fernández-Lobato M, et al. Methods Mol. Biol. 898, 219-34, (2012)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?