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H5529 Sigma

Heregulin-α, EGF Domain human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Synonym: HRG-α

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Description

Analysis Note

The bioactivity is tested in culture using a proliferation assay with the human cell line MCF-7.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 50 μg bovine serum albumin per 1 μg as a carrier protein.

Biochem/physiol Actions

Heregulin (HRG) is the human homologue to the neu differentiation factor (NDF) in rat. The heregulin family members contain one EGF-like motif and an IgD-like motif in the extracellular domain. They bind to ErbB-2, ErbB-3, and ErbB-4 (receptors closely related to EGFR). HRG-α and HRG-β isoforms differ slightly in the EGF domain due to alternate splicing. HRG-β isoforms are further subdivided into β1, β2, and β3 isoforms, which show identical binding and activation characteristics. Both α and β HRG isoforms bind to ErbB-3 and ErbB-4 homodimers, but not directly to ErbB-2. HRG-α binding to ErbB-3 and ErbB-4 is reported to be approximately 100-fold weaker than that of HRG-β. When ErbB-2 is combined into a heterodimer with ErbB-3 or ErbB-4, the binding affinities of both α and β isoforms are substantially improved. HRGs are mitogenic for Schwann cells in culture and weakly to moderately mitogenic for a variety of epithelial cells, including mammary, ovarian, lung, and gastric cells. HRGs inhibit proliferation and induce differentation in some tumor cell lines, such as certain mammary tumor cells, which are arrested at the G2M phase. HRGs also induce expression of acetylcholine receptors and possibly other molecules in muscle cells at newly formed neuromuscular synapses, suggesting they may play a role in neuromuscular synapse maturation and maintenance.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

EGFR Pathway

Background EGF Receptors & Ligands    Receptor Activation EGFR Signaling    Ras/Raf Signaling Cascade    Phosphatidylinositol 3-kinase/Akt Signaling Cascade    Signal Transducers and Activators of Tr...
Keywords: Apoptosis, Cancer, Cell proliferation, Central Nervous System, Clinical, Degradations, Diseases, Electron spin resonance, Gastrointestinal, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Morphogenesis, Transcription, Transduction

Peer-Reviewed Papers
15

References

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