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H5625 Sigma

Hexokinase from Saccharomyces cerevisiae

Type C-300, ammonium sulfate suspension, 130-250 units/mg protein (biuret)

Synonym: ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast



Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Carbohydrate Metabolism, Cell Biology,
type   Type C-300
form   ammonium sulfate suspension
foreign activity   ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
storage temp.   2-8°C
Gene Information   bakers yeast ... HXK1(850614), HXK2(852639)



A mixture of isoenzymes.

Unit Definition

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 containing 2.5 mM phosphate buffer, pH 7.0, and 0.25 mM EDTA

Biochem/physiol Actions

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

Price and Availability

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

WGK Germany 
Protocols & Articles


Enzymatic Assay of Hexokinase

This procedure applies to most products1 that have a specification for Hexokinase determination by enzymatic determination.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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H4502 Hexokinase from Saccharomyces cerevisiae, Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

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