• USA Home
  • H5654 - Hemocyanin, succinylated from Megathura crenulata (keyhole limpet)

H5654 Sigma

Hemocyanin, succinylated from Megathura crenulata (keyhole limpet)

lyophilized powder



Related Categories Antibodies, Antibody Purification and Characterization, Carrier Proteins, Supplementary Products
form   lyophilized powder
mol wt   mol wt 8,000-9,000 kDa
storage temp.   2-8°C


Preparation Note

Prepared by modification of KLH with succinic anhydride.


Reconstitute with 2 mL deionized water to yield an opalescent solution of KLH at 5 mg/ml in sodium phosphate buffer, pH 7.4, containing 0.32 M NaCl and 20 mM sucrose.
Reconstituted protein solution may be stored up to two months at –20°C.


This specially prepared, chemically modified form of KLH is highly water-soluble and provides a large number of available carboxyl groups for conjugation to hapten. The conjugation of succinylated KLH to an amine group-containing hapten (e.g., peptide), mediated by water-soluble carbodiimide as cross-linking reagent, proceeds favorably between the newly introduced carboxyl groups of KLH and the amine groups of the hapten.

Physical form

Lyophilized with stabilizing buffer

Price and Availability

Amplified Detection
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?