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H5654 Sigma

Hemocyanin, succinylated from Megathura crenulata (keyhole limpet)

lyophilized powder

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, Carrier Proteins, Supplementary Products
form   lyophilized powder
mol wt   mol wt 8,000-9,000 kDa
storage temp.   2-8°C

Description

Preparation Note

Prepared by modification of KLH with succinic anhydride.

Reconstitution

Reconstitute with 2 mL deionized water to yield an opalescent solution of KLH at 5 mg/ml in sodium phosphate buffer, pH 7.4, containing 0.32 M NaCl and 20 mM sucrose.
Reconstituted protein solution may be stored up to two months at –20°C.

Specificity

This specially prepared, chemically modified form of KLH is highly water-soluble and provides a large number of available carboxyl groups for conjugation to hapten. The conjugation of succinylated KLH to an amine group-containing hapten (e.g., peptide), mediated by water-soluble carbodiimide as cross-linking reagent, proceeds favorably between the newly introduced carboxyl groups of KLH and the amine groups of the hapten.

Physical form

Lyophilized with stabilizing buffer

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

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