Anti-phospho-Histone H2AX (pSer¹³⁹) antibody produced in rabbit

Immunogen

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.

synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer¹³⁹).

General description

Histones (H1, H2B, H2A, H3 and H4) regulate the packaging of eukaryotic DNA into repeating nucleosome units that are folded in higher-order chromatin fibers ¹. Phosphorylation of serine 139 in the histone H2AX subtype is induced by double-stranded breaks in DNA. H2AX histone has also been implicated in DNA repair mechanisms following double-strand breaks ². Anti-phospho-Histone H2AX [pSer139] binds to histone H2AX phosphorylated on Ser¹³⁹ (γ-H2AX) by immunoblotting (15 kDa). Staining of [pSer¹³⁹] histone H2AX in immunoblotting is specifically inhibited with the [pSer¹³⁹] histone H2AX immunizing peptide (human, amino acids 134-142). No inhibition is observed with the respective non-phosphorylated histone H2AX peptide (human, amino acids 134-142). The antibody product is also reactive in mice.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-phospho-Histone H2AX (p(Ser^139M)) antibody is suitable for use in western blot (1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine) and microarray. The antibody may also be used in immunoblot assays.