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H6020 Sigma

Hybridoma Enhancing Supplement Hybri-Max from murine thymoma cell line

liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym: Conditioned Medium from murine thymoma cell line

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Hybridoma Media Supplements, Hybridoma Platform, Hybridoma Reagents,
sterility   sterile-filtered
form   liquid
impurities   Mycoplasma, tested
  endotoxin, tested
suitability   suitable for cell culture
  suitable for hybridoma
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Cell line virus testing:
Murine Antibody Production (MAP) testing for Sendai, MHV, PVM, Reo 3, GDVII, Ectomelia, MVM, Polyoma, LDVM, M. Ad. EDIM MCMV, LCM, Hantaan.

Application

Use at a concentration of 10% in complete medium containing FBS (10%) or CPSR-3. Optimal conditions must be determined for specific applications.

Other Notes

Conditioned medium from a proprietary murine thymoma cell line grown in RPMI-1640 with 15% FBS.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the storage temperature of Product No. H6020, Hybridoma Enhancing Supplement Hybri-Max™?
The product may be stored at -20°C. Avoid repeated freeze-thaw cycles by storing working aliquots and thawing only once.
There is a precipitate in the Product No. H6020, Hybridoma Enhancing Supplement Hybri-Max™ when I thawed it. Can I still use the product?
The precipitate is not detrimental to the product and may be reversed when warmed to 37ºC.
What is the endotoxin level of the Product No. H6020, Hybridoma Enhancing Supplement Hybri-Max™?
The specification for the product is an Endotoxin level that is less than or equal to 10 ng/mL.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles
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