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H6024 Sigma

Hoechst Stain solution

Synonym: Bisbenzimide H 33258

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Description

Components

Contains 0.5μg/ml Hoechst bisbenzimide 33258 fluorochrome stain and thimerosal.

Application

A fluorescent DNA stain qualified for use in Mycoplama staining.

General description

Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells. It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Mycoplasma Detection/Elimination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest concerns are mycoplasma contamination. Although mycoplasma do not usually kill contaminated cel...
Don Finley
BioFiles v7 n1, 2011, 14–15
Keywords: Eliminations, Metabolism, Polymerase chain reaction

Protocols

Testing for Mycoplasma by Indirect DNA Stain

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction

Peer-Reviewed Papers
15

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