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H6390 Sigma

Hemin−Agarose

Type I, saline suspension

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Properties

Related Categories Affinity Chromatography, Molecular Biology, Protein Chromatography, Proteomics, Specialty Resins More...
biological source   hemin bovine
type   Type I
form   saline suspension
extent of labeling   ≥4 μmol (per ml)
matrix   Cross-linked 4% beaded agarose
matrix activation   epoxy
matrix attachment   carboxyl
matrix spacer   12 atoms
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

25 mL in glass bottle

Physical form

Suspension in 0.5 M NaCl containing 0.02% thimerosal

Application

Hemin Type I-agarose is an agarose conjugate in saline suspension used in affinity chromatography, protein chromatography and specialty resins. Hemin-agarose has been used to evaluate protection against invasion by Gram-negative bacteria.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Where can I find a protocol for using Product H6390, Hemin-Agarose?
A reference that describes both the preparation and use of hemin-agarose is Analytical Biochemistry, 121, 244-250 (1982).
Can organic solvents be used with Product H6390, Hemin-Agarose?
Yes, up to 20% ethanol, acetone or neat dimethylformamide may be used.
What is the recommended negative control to use with Product H6390, Hemin-Agarose?
Product 4B200 (Sepharose 4B) is a good negative control to use for H6390.
What is the recommended pH range for Product H6390, Hemin-Agarose?
The pH range that the hemin agarose can be used at (and at which the hemin is expected to be stable) is 6.5-9.0.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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