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H6533 Sigma

Monoclonal Anti-HA−Peroxidase antibody produced in mouse

clone HA-7, purified immunoglobulin, lyophilized powder

Synonym: Anti-HA, Anti-Influenza Hemagglutinin, Monoclonal Anti-HA

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, H1-HE, HA,
biological source   mouse
antibody form   purified immunoglobulin
clone   HA-7, monoclonal
form   lyophilized powder
packaging   vial of 0.5 mL
concentration   5 - 11 mg/mL
application(s)   western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent
conjugate   peroxidase conjugate
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Suitable for immunoblotting

Features and Benefits

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General description

The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.

Immunogen

synthetic peptide corresponding to amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH.

Preparation Note

Reconstitute in .5mL of distilled water.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal.

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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