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H6908 Sigma

Anti-HA antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-HA

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, H1-HE,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   immunoprecipitation: 2.5-4 μg/test using HA-tagged fusion protein from cell lysates
  indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells
  western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts
conjugate   unconjugated
Featured Industry   Research Pathology
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Immunogen

synthetic peptide corresponding to amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

Anti-HA antibody is suitable for use in immunoprecipitation and western blot.
It is also suitable for indirect immunofluorescence (10-20μg/mL using HA-tagged fusion protein transfected cells), immunoprecipitation (2.5-4μg/test using HA-tagged fusion protein from cell lysates), and western blot (0.5-0.8μg/mL using HA-tagged fusion protein transfected cell extracts).

General description

Hemagglutinin (HA) is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection . The HA gene is often used as an affinity tag for target proteins in recombinant expression vector systems. The HA-tagged proteins produced from such systems yield stable fusion products that do not interfere with the function and distribution of target proteins
Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins. The product may detect cross-reacting bands in certain mammalian cells. The immunizing HA peptide (Product No.I2149) specifically inhibits the staining of the HA-tagged protein band.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

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