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H7033 Sigma

PerfectHyb Plus Hybridization Buffer

for Northern and Southern blotting, solution



Features and Benefits

• Fast and easy-to-use
• Up to 20x more sensitive than conventional buffers
• Doesn’t require blocking steps
• Compatible with neutral and positively-charged membranes
• Compatible with chemiluminescent, colorimetric , and DIG detection assays
• Compatible with radioactive and non-radioactive probes
• Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

General description

Sigma’s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.

Other Notes

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.


Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Legal Information

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations


PerfectHyb™ Plus

Product Description Precautions and Disclaimer Storage/Stability Preparation Instructions Procedure Troubleshooting Guide Materials Reference
Keywords: AGE, Buffers, Electrophoresis, Gel electrophoresis, Molecular biology, Northern blot, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Southern blot

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Peer-Reviewed Papers


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