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Hematopoietic Stem Cell Protocols

  •  ISBN-10 0-89603-812-2

  •  ISBN-13 978-0-89603-812-7

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publication info   C.Klug and C. Jordan, ed., Humana Press, 2002, 344 pp., hard cover
mfr. no.   Humana

Description

General description

Stem-cell investigators describe key methods for investigating hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The approaches employ molecular and cell biology techniques, along with animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. These techniques for studying a variety of stem cell topics, including blood formation, blood-based disease, and blood cancers adds knowledge that is crucial in the development of clinical medicine.

Table of Contents

Isolation and Analysis of Hematopoietic Stem Cells from Mouse Embryos
The Purification of Mouse Hematopoietic Stem Cells at Sequential Stages of Maturation
Flow Cytometry and Immunoselection of Human Stem Cells
Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Noninvasive Measurement of Hematopoietic Stem Cell Cycling and Turnover by In Vivo Bromodeoxyuridine Incorporation
Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in Cell Cycle
Hematopoietic Colony-Forming Cells
Long-Term Culture-Initiating Cell Assays for Human and Murine Cells s
The Cobblestone-Area-Forming Cell Assay
Colony Forming Unit in Spleen: An Assay for Pluripotent Myelopoietic Stem Cells
Intrathymic Injection for Analysis of T-Cell Progenitor Activity
Quantitation of Murine and Human Hematopoietic Stem Cells by Limiting-Dilution Analysis in Competitively Repopulated Hosts
Ex Vivo Expansion of Human and Murine Hematopoietic Stem Cells
Hematopoietic Development of ES Cells in Culture
Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses
Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells
Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Progenitor Cells
Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
Identification of Differentially Expressed Genes in Sorted Cell Populations by Two-Dimensional Gene-Expression Fingerprinting

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