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H8032 Sigma

Hanahan’s Broth (SOB Medium)

powder microbial growth medium

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

20 g/L Tryptone
5 g/L Yeast Extract
2.4 g/L MgSO4
0.5g/L NaCl
0.186 g/L KCl

Features and Benefits

Hannahan’s Broth (SOB) powder provides:
• Standard formulation
• An inexpensive alternative to liquid

General description

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Reconstitution

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Price and Availability


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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Protocols

Competent Cells for Transformation

Transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. It was first reported in Streptococcus pneumoniae by ...
Keywords: Antibiotics, Cloning, Environmental, Gene expression, Genetic, Molecular biology, Peptide synthesis, Polymerase chain reaction, Transfection, transformation

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

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