• USA Home
  • H8032 - Hanahan’s Broth (SOB Medium)

EMAIL THIS PAGE TO A FRIEND
H8032 Sigma

Hanahan’s Broth (SOB Medium)

powder microbial growth medium

Purchase

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

20 g/L Tryptone
5 g/L Yeast Extract
2.4 g/L MgSO4
0.5g/L NaCl
0.186 g/L KCl

Features and Benefits

Hannahan’s Broth (SOB) powder provides:
• Standard formulation
• An inexpensive alternative to liquid

General description

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Reconstitution

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol.  Generally: LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics. LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content. Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium. SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells. SOC is used for initial growth of competent cells and the transformation procedure.
Show more questions
Protocols & Articles

Articles

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Protocols

Competent Cells for Transformation

Transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. It was first reported in Streptococcus pneumoniae by ...
Keywords: Antibiotics, Cloning, Environmental, Gene expression, Genetic, Molecular biology, Peptide synthesis, Polymerase chain reaction, Transfection, transformation

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

S1797 SOC Medium, For use in transformation
L7658 LB Broth (Lennox), EZMix powder microbial growth medium
L3022 LB Broth (Lennox), Powder microbial growth medium
T5574 Terrific Broth, Liquid microbial growth medium
CMC0001 SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production
CMC0003 SIG10 HIGH Electrocompetent Cells, for protein expression and DNA plasmid production
CMC0007 SIG10 5a Chemically Competent Cells, for DNA plasmid production
CMC0014 BL21(DE3) Chemically Competent Cells, for protein expression
CMC0017 OverExpress C41(DE3) Chemically Competent Cells, for the highest protein expression

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?