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H8142 Sigma

Hybridoma Enhancing Supplement from murine macrophage-like cell line

liquid, sterile-filtered, suitable for hybridoma

Synonym: Conditioned Medium from murine macrophage-like cell line



Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Hybridoma Media Supplements, Hybridoma Platform, Hybridoma Reagents,
sterility   sterile-filtered
form   liquid
impurities   Mycoplasma, tested
  endotoxin, tested
suitability   suitable for hybridoma
shipped in   dry ice
storage temp.   −20°C


Analysis Note

Cell line viral testing:
Murine Antibody Production (map) testing for Sendai, MHV, PVM, Reo3, GDVII, Ectomelia, MVM, Polyoma, LDMV, M. Ad, EDIM, MCMV, LCM, Hantaan.
BVD virus by fluorescent antibody stain


Use at a concentration of 10% in complete medium containing FBS (10%) or CPRS-3. Optimal conditions must be determined for specific applications.


Avoid repeated freezing and thawing. If entire contents are not used, store frozen in working aliquots. Precipitation may occur when stored. This is not detrimental to the product and may be reversed when warmed to 37 °C.

Other Notes

Conditioned medium from a proprietary murine macrophage-like cell line grown in IMDM with 2% FBS.

Price and Availability

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Cell Quantification

For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation

Cryopreservation of Cell Lines

The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions

Resuscitation of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE

Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion

Subculture of Semi-Adherent Cell Lines

Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture

Subculture of Suspension Cell Lines

In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors

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