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H8629 Sigma

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae)

lyophilized powder, hexokinase ≥65 units/mg protein, glucose-6-phosphate dehydrogenase ≥75 units/mg protein (biuret)

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Properties

Related Categories Carbohydrate Metabolism, Cell Biology, General Metabolic Enzymes, Glycolysis, Glycolysis Enzymes,
form   lyophilized powder
purified by   crystallization
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Mixture of hexokinase and glucose-6-phosphate dehydrogenase

Application

Useful for determination of glucose and ATP.

General description

Enzyme Commission numbers 2.7.1.1 + 1.1.1.49

Packaging

500 units in glass bottle

Package sizes are based on hexokinase units.

5000 units in poly bottle

Unit Definition

Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Can Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be used for the determination of glucose?
A method for the determination of glucose using hexokinase and glucose 6-phosphate dehydrogenase is described in "Methods of Enzymatic Analysis" 3rd ed., Vol. VI, p. 163.
What units are the package sizes of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, based upon?
This product is packaged on the basis of hexokinase units. To view this information on the Product Detail Page, please click on the following link: http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/H8629
What is the stability of a stock solution of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?
Stock solutions prepared in water or 10 mM potassium phosphate buffer pH 8.0 lose approximately 1% activity per month when stored at 4°C.
Does Sigma offer any other product which can be used in place of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?
Yes. We offer Product No. G3293 (Glucose Assay Reagent), which is a component of the GAHK20 (Glucose Assay Kit).
How should Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be reconstituted?
Prior to determining the activity of this enzyme, Sigma reconstitutes it in 250 mM glycylglycine buffer, pH 7.4.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Protocols

Enzymatic Assay of Myokinase (EC 2.7.4.3)

Replaces OP SP-ADP01. New document assigned to conform to current document numbering system. Refer to CR SOP-DEK-ENZ47.
Keywords: Extinction coefficient, Molecular biology

Peer-Reviewed Papers
15

References

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Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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