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H8651 Sigma

HEPES sodium salt

BioXtra, ≥99.0% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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Properties

Related Categories BioXtra Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
InChI Key   RDZTWEVXRGYCFV-UHFFFAOYSA-M
Quality Level   ELITE
product line   BioXtra
assay   ≥99.0% (titration)
impurities   ≤0.005% Phosphorus (P)
  ≤0.1% Insoluble matter
pH   10.0-12.0 (1 M in H2O)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
solubility   H2O: 1 M clear, colorless
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
absorption   A1M/260, H2O ≤0.2
  A1M/280, H2O ≤0.2
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

Packaging

25, 100, 500 g in poly bottle

Application

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Peer-Reviewed Papers
15

References

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