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H8889 Sigma

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

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Properties

Related Categories ACCUSPIN and Histopaque, Cell Biology, Cell Culture, Cell Separation, Density Gradient Media,
Quality Level   PREMIUM
sterility   sterile-filtered
product line   BioReagent
form   liquid
impurities   endotoxin, tested
density   1.077 g/mL at 25 °C(lit.)
suitability   suitable for hybridoma
storage temp.   2-8°C

Description

Application

Used to create a density medium for the purification of lymphocytes and other mononuclear cells. Histopaque®-1077 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/ml. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell mediated lympholysis and for human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step prior to enumeration of T-, B-, and ‘null’ lymphocytes.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Histopaque® Troubleshooting Guide

The following recommendations for troubleshooting the use of Histopaque and ACCUSPIN™ in cell separation techniques have been compiled by Sigma-Aldrich® Technical Services. These are based on observa...
Mark Frei
BioFiles v6 n5, 14–16
Keywords: Adhesion, Anticoagulants, Cell culture, Centrifugation, Coagulation, Culture media, Sample preparations, Separation, Sterilizations

Peer-Reviewed Papers
15

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08168 Timestrip Plus 8 °C

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