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H9268 Sigma

Hexadimethrine bromide

≥94% (titration)

Synonym: 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, Polybrene

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Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for RNAi, Life Science Reagents for Transfection, Research Essentials More...
assay   ≥94% (titration)
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

5, 10, 50, 100 g in poly bottle

Application

Hexadimethrine bromide can be used to transfect mammalian cells with DNA. It can be used to increase the efficiency of lipofection transfections.
Hexadimethrine bromide has been used for lentivirus infection in cells. It has been used for infection of cells with retroviral supernatant.

General description

Hexadimethrine bromide is a quaternary ammonium compound which works as a heparin antagonist. It is also involved in the transformation of low molecular weight DNA.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TQ2655000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Is Product H9268, Hexadimethrine bromide, the same thing as Polybrene?
Yes, Polybrene (registered trade-mark of Abbott) and 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, are Synonyms.  Synonyms are listed on the Product Bulletin at the following link.  http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/h9268bul.pdf  
What is the molecular weight of Product H9268, Hexadimethrine bromide?
According to the information from our supplier, the molecular weight of this product is 4,000-6,000.  The Merck Index provides a general MW range for the chemical of 5,000 - 10,000. General product information can be accessed on the Product Information Sheet by accessing the following link:   http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/2/h9268pis.Par.0001.File.tmp/h9268pis.pdf 
What is the solubility of Product H9268, Hexadimethrine bromide?
Soluble in water up to 10%.  This information is found on the Product Information Sheet which may be accessed at the following link.  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/2/h9268pis.Par.0001.File.tmp/h9268pis.pdf
How do I sterilize Product H9268, Hexadimethrine bromide?
Hexadimethrine bromide can be filtered sterilized or autoclaved.
What is the solution stability of Product H9268, Hexadimethrine bromide?
Stock solutions in 0.9% saline solution at 1 mg/mL are stable at least one year stored in the refrigerator.  Stability information is found on the Product Bulletin.  This can be accessed at the following link.  http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/h9268bul.pdf
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Sequences, Transcription, Transduction, Vectors

Protocols

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Related Content

MISSION® LentiPlex® Pooled shRNA Library Screening in Mammalian Cells [VIDEO]

The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The TRC 1.0 library consists of over 80,000 shRNA constructs from the TRC colle...
Keywords: Amplification, Antitumor agents, Cancer, Oncology, Sequencing

Peer-Reviewed Papers
15

References

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