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H9658 Sigma

Monoclonal Anti-HA antibody produced in mouse

clone HA-7, ascites fluid

Synonym: Anti-HA, Anti-Influenza Hemagglutinin, Monoclonal Anti-HA

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, H1-HE,
biological source   mouse
antibody form   ascites fluid
clone   HA-7, monoclonal
contains   15 mM sodium azide
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Immunogen

synthetic peptide corresponding to amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

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RIDADR 
NONH for all modes of transport
WGK Germany 
2

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
What is the recommended dilution of Product H9658 - HA antibody for immunoprecipitation and for indirect immunostaining?
H9658 - Monoclonal Anti-HA antibody produced in mouse is the ascites fluid. It is not purified. We only test this product in western blotting.  This product contains 15-55 mg/ml total protein with 4-7 mg/ml specific antibody.  H3663 - Monoclonal Anti-HA antibody produced in mouse as the purified immunoglobulin. We test this product for immunoprecipitation using 0.5-2.0 μg and for immunocytochemistry using 1.0-2.0 μg/mL. These ranges would be a good starting concentration. In order to obtain best results in different techniques and preparations, we recommend determining optimal working dilution by titration test.
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