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H9886 Sigma

p-Hydroxybenzoate Hydroxylase from Pseudomonas sp.

lyophilized powder, ~20 units/mg solid

Synonym: 4-Hydroxybenzoate, NADPH:oxygen oxidoreductase (3-hydroxylating)

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Description

Application

This enzyme is useful for enzymatic determination of choline esterase when coupled with protocatechuate 3, 4-dioxygenase.

Unit Definition

One unit will hydroxylate 1.0 μmole of p-hydroxybenzoate to protocatechuate per min at pH 8.2 at 37 °C in the presence of NADPH.

Physical form

Contains mannitol and stabilizers

Physical properties

Michaelis constants : 2.0 x 10-5M (p-Hydroxybenzoate), 4.0 x 10-5M (NADPH)
Structure : One mol of FAD per mol of enzyme
Inhibitors : Ag+, Hg++, PCMB, SDS
Optimum pH : 7.7 - 7.9
Optimum temperature : 35oC
pH Stability : pH 5.0 - 7.5 (25oC, 72hr)
Thermal stability : below 40oC (pH 6.0, 15min)

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Assay Procedure for p-Hydroxybenzoate Hydroxylase

One unit causes the oxidation of one micromole of NADPH per minute under the conditions described below.
Keywords: Extinction coefficient, Oxidations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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