HT20 Sigma

Iron Stain Kit

Synonym: Prussian Blue Test Kit



Related Categories Cell Biology, Hematology and Histology, Histology Special Stains More...
Quality Level   GMP-IVD
shelf life   Expiry date on the label.


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Deposits of loosely bound iron in erythrocytes, bone marrow, or tissue stain an intense blue when treated with acid ferrocyanide according to the well-known Prussian blue reaction.

Price and Availability

Tissue Diagnostics

All labs need water

Biomedical Applications

Kit component only


Product #

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Hydrochloric acid solution 250 mL SDS    
Potassium ferrocyanide solution 250 mL SDS    
Pararosaniline solution 30 mL SDS    
Safety & Documentation

Safety Information

Signal word 
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
UN 3316 9
WGK Germany 
Flash Point(F) 
51.8 °F
Flash Point(C) 
11 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
Can I buy the reagents in Iron Stain Kit, Product HT20, separately?
The reagents included in this kit are not available for separate purchase.
Is EDTA an acceptable anticoagulant for use with the Iron Stain Kit, Product HT20?
Blood and bone marrow samples should be drawn without the use of EDTA as an anticoagulant. The EDTA chelates enough Iron to interfere with adequate staining. We advise using heparinized blood or freshly drawn blood which has not been anticoagulated.
Is it necessary to use the counterstain to view tissue Iron when using the Iron Stain Kit, Product HT20?
Iron in tissue samples (used for research) can be visualized without the Pararosaniline counterstain, which is used to allow improved visualization of the tissue.
Can the Iron Stain Kit, Product HT20, be used to stain cultured cells, rather than blood or bone marrow?
This kit can be used to stain cultured mammalian cells, which should be treated as blood or bone marrow and follow instructions given for specimen collection in the Package Insert.  We advise using some sort of positive control.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers


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