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In Vitro Neurochemical Techniques

  •  ISBN-10 0-89603-509-3

  •  ISBN-13 978-0-89603-509-6

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publication info   A.A. Boulton, et al., ed., Humana Press, 1999, 416 pp., hard cover
mfr. no.   Humana

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Techniques range from isolation of neuronal genes and their expression to analysis of receptor-ligand interactions, to characterization of consequences of receptor activation. Methods include electrophysiological techniques to explore functional properties of receptors present in membranes of excitable cells and those to isolate novel genes central to neurobiological processes. Others cover measuring changes in gene expression, rapid identification of gene polymorphisms, and identification and characterization of second messenger pathways.

Table of Contents

Characterization of Receptors by Radiolabeled Ligand Binding Techniques.
Electrophysiological Studies on Receptors In Vitro,
Subtractive Hydridization: A Method to Identify Novel Genes Induced During Neuronal Programmed Cell Death.
Messenger RNA Differential Display: An Unbiased Tool to Uncover Alterations in Gene Expression Underlying Biological Processes,
Localization of mRNAs in Brain Sections by in situ Hybridization Using Oligonucleotide Probes.
Multiprobe Oligonucleotide Solution Hybridization for the Determination of Relative mRNA Levels,
Competitive RT-PCR to Quantitate Steady-State mRNA Levels.
A Novel PCR-RFLP Detection Method Using an Optimized Set of Restriction Enzymes,
Western Blot Analysis,
The Phosphoinositide Signal Transduction System,
Protein Phosphorylation. Immunoprecipitation of Receptors.
Index.

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