I1146 Sigma

R3 IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture



Related Categories Cell Culture, Growth Factor Analogs, Growth Factors and Cytokines, Human recombinant IGF proteins and receptors, Insulin-like Growth Factors (IGF),
recombinant   expressed in E. coli
assay   ≥85% (HPLC)
form   lyophilized powder
quality   endotoxin tested
suitability   suitable for cell culture
storage temp.   2-8°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Product stimulates protein synthesis in L6 myoblasts.

Other Notes

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Preparation Note

Prepared from 0.1 M acetic acid


R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Price and Availability

All labs need water
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?
Here is the link to the UniProt page for human insulin-like growth factor 1, amino acids 49-118:[49-118] That is, the entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118. Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is: GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA Product I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1: MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA Note that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1:
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
Protocols & Articles


Insulin-like Growth Factors (IGF)

Insulin-like growth factors (IGF-I and IGF-II) are mitogenic and anabolic peptides structurally homologous to insulin. IGF-I and -II are single polypeptide chains of approximately 7.5 kDa comprised o...
Jennifer Fries
BioFiles 2009, 4.5, 12.
Keywords: Adhesion, Apoptosis, Biofiles, Cancer, Growth factors, Hormones, Infrared spectroscopy, Ligands, Transduction, transformation

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

I8779 Insulin-like Growth Factor-I from mouse, IGF-I, recombinant, expressed in E. coli, lyophilized powder, cell culture tested
08168 Timestrip Plus 8 °C
I9278 Insulin solution human, Chemically defined, recombinant from Saccharomyces cerevisiae, sterile-filtered, BioXtra, suitable for cell culture
I3769 Insulin-like Growth Factor-I human, IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?