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I1146 Sigma

R3 IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Properties

Related Categories Cell Culture, Growth Factor Analogs, Growth Factors and Cytokines, Reagents and Supplements
recombinant   expressed in E. coli
assay   ≥85% (HPLC)
form   lyophilized powder
quality   endotoxin tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Product stimulates protein synthesis in L6 myoblasts.

Other Notes

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Preparation Note

Prepared from 0.1 M acetic acid

Application

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

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All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Insulin-like Growth Factors (IGF)

Insulin-like growth factors (IGF-I and IGF-II) are mitogenic and anabolic peptides structurally homologous to insulin. IGF-I and -II are single polypeptide chains of approximately 7.5 kDa comprised o...
Jennifer Fries
BioFiles 2009, 4.5, 12.
Keywords: Adhesion, Apoptosis, Biofiles, Cancer, Growth factors, Hormones, Infrared spectroscopy, Ligands, Transduction, transformation

Peer-Reviewed Papers
15

References

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