Synonym: IPTG solution
|Related Categories||Biochemicals and Reagents, Core Bioreagents, Detergents, Detergents A to M, Detergents by Applications,|
|grade||for molecular biology|
|shipped in||dry ice|
IPTG Solution is composed of 200 mM IPTG in molecular biology grade water.
Suitable for induction of recombinant protein expression in systems controlled by the lac repressor.
Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter.
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for ß--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.
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The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens. Hansen LH, Knudsen S, and Sorensen SJ Curr. Microbiol. 36, 341-347, (1998)
Quantitative, non-disruptive monitoring of transcription in single cells with a broad-host range GFP-luxCDABE dual reporter system. Benedetti IM, de Lorenzo V, and Silva-Rocha R PLoS ONE 7(12), e52000, (2012)
Optimization of TNF-α overexpression in Escherichia coli using response surface methodology: Purification of the protein and oligomerization studies. Papaneophytou CP and Kontopidis GA Protein Expr. Purif. 86(1), 35-44, (2012)
Production of soluble recombinant proteins in Escherichia coli: effects of process conditions and chaperone co-expression on cell growth and production of xylanase. Jhamb K and Sahoo DK Bioresour. Technol. 123, 135-43, (2012)
Two systems for conditional gene expression in Myxococcus xanthus inducible by isopropyl-β-D-thiogalactopyranoside or vanillate. Iniesta AA, García-Heras F, Abellón-Ruiz J, et al. J. Bacteriol. 194(21), 5875-85, (2012)
Cloning analysis of ferritin and the cisplatin-subunit for cancer cell apoptosis in Aplysia juliana hepatopancreas. Zhu B, Huang L, and Huang HQ Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 156(2), 95-103, (2012)
Expression, purification and structural analysis of recombinant rBdh-2His₆, a spermadhesin from buck (Capra hircus) seminal plasma. Nascimento AS, Cajazeiras JB, Nascimento KS, et al. Reprod. Fertil. Dev. 24(4), 580-7, (2012)
Reduction of N-terminal methionylation while increasing titer by lowering metabolic and protein production rates in E. coli auto-induced fed-batch fermentation. Xu J, Qian Y, Skonezny PM, et al. J. Ind. Microbiol. Biotechnol. 39(8), 1199-208, (2012)
Multigram synthesis of isobutyl-β-C-galactoside as a substitute of isopropylthiogalactoside for exogenous gene induction in mammalian cells. Liu L, Abdel Motaal B, Schmidt-Supprian M, et al. J. Org. Chem. 77(3), 1539-46, (2012)
Evidencing the role of lactose permease in IPTG uptake by Escherichia coli in fed-batch high cell density cultures. Fernández-Castané A, Vine CE, Caminal G, et al. J. Biotechnol. 157(3), 391-8, (2012)
A two-step fluorescence-activated cell sorting approach to isolate genetically modified mammalian cells with isopropyl-beta-D-thiogalactoside (IPTG)-inducible gene expression. Mok MT and Henderson BR Cytometry. A 81(2), 101-4, (2012)
Molecular Cloning, A Laboratory Manual Sambrook, J.F. and Russell, D.W. Molecular Cloning: A Laboratory Manual 2nd ed.,, (1989)
Current Protocols in Molecular Biology Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, L.A. and Struhl, K. Curr. Protoc. Mol. Biol.
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