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I1768 Sigma

In Situ Hybridization: A Practical Approach

  •  ISBN-10 0-19-963327-4

  •  ISBN-13 978-0-19-963327-2



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publication info   D.G. Wilkinson, ed., IRL Press at Oxford University Press, Oxford, England, 1992, 163 pp., soft cover
mfr. no.   Oxford University Press


General description

Step-by-step protocols are presented for the preparation of cloned DNA, RNA and oligonucleotide probes, with both radioactive and non-radioactive labels, and their hybridization to mRNA, viral DNA and chromosomal DNA.

Table of Contents

1. The Theory and Practice of In Situ Hybridization, D.G. Wilkinson
2. In Situ Hybridization to Cellular RNA with Radiolabelled RNA Probes, L.M. Angerer and R.C. Angerer
3. In Situ Hybridization with Oligodeoxyribonucleotide Probes, W. Scott Young, III
4. In Situ Hybridization with Biotinylated Probes, P. Emson and M.J. Gait
5. Whole Mount In Situ Hybridization in Drosophila, D. Tautz, M. Hulskamp, and R.J. Sommer
6. Whole Mount In Situ Hybridization of Vertebrate Embryos, D.G. Wilkinson
7. Simultaneous Detection of Cellular RNA and Proteins, M. Brahic and S. Ozden
8. In Situ Hybridization at the Electron Microscope Level, M. Binder
9. Detection of Virus in Infected Human Tissue by In Situ Hybridization, F.A. Lewis and M. Wells
10. In Situ Hybridization to Chromosomes with Biotinylated Probes, E. Viegas-Pequignot

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