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I1882 Sigma

Insulin from bovine pancreas

γ-irradiated, BioXtra, suitable for cell culture, potency: ≥20 units/mg (USP units), lyophilized powder

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines, Growth Factors and Hormones, Hormones,
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
potency   ≥20 units/mg (USP units)
suitability   suitable for cell culture
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Insulin is used as a growth factor in many mammalian cell culture systems.

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Reconstitution

To prepare 10 mg/ml stock solution, add 10 ml of acidified H2O (pH ≤2) - prepared by addition of glacial acetic acid (approx. 0.1 ml).

Physical form

powder-0 °C; solution stable 2-8 °C, 1 year

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
What is the comparison between International Units (IU) and USP units for insulin?
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
What can be used to make a solution of insulin?
Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 µL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516; here is the link to the detail page for that product: http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/I0516
How can I store the insulin solution?  How long?
The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
What is the amino acid sequence for Product I1882, Insulin from bovine pancreas?
The sequence and structure of insulin can be found on the product information sheet; see the link below: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/2/i1882pis.Par.0001.File.tmp/i1882pis.pdf
How many units of insulin are present in one vial of Product I1882, Insulin from bovine pancreas?
Product No. I1882 has a potency specification of not less than 2520 USP units per vial. For a vial that contains (nominally) 100 mg, that works out to 25.2 USP units/mg.  The potency in terms of units per vial can be found the on lot-specific Certificate of Analysis. This document may be obtained through our Advanced Search Option:  http://www.sigmaaldrich.com/catalog/search/AdvancedSearchPage?TabSelection=RelatedInformation
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles
Peer-Reviewed Papers
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References

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