I1882 Sigma

Insulin from bovine pancreas

γ-irradiated, BioXtra, suitable for cell culture, potency: ≥25 units/mg (USP units), lyophilized powder

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines, Growth Factors and Hormones, Hormones,
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
potency   ≥25 units/mg (USP units)
suitability   suitable for cell culture
storage temp.   −20°C

Description

Frequently Asked Questions

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Application

Insulin is used as a growth factor in many mammalian cell culture systems.

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Reconstitution

To prepare 10 mg/ml stock solution, add 10 ml of acidified H2O (pH ≤2) - prepared by addition of glacial acetic acid (approx. 0.1 ml).

Physical form

powder-0 °C; solution stable 2-8 °C, 1 year

Price and Availability

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