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I1884 Sigma

Insulin-transferrin-sodium selenite media supplement

γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture

Synonym: ITS Supplement

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Properties

Related Categories Cell Culture, Hybridoma Media Supplements, Hybridoma Platform, ITS and Other Supplements, Reagents and Supplements,
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
suitability   suitable for cell culture
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite.

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Quantity

Each vial sufficient to prepare 5 liters of medium.

Application

Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.

INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.

TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.

SELENIUM is an essential trace element normally provided by Serum.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Transferrin in Cell Culture

Importance and uses of transferrins in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Cell culture, Culture media, Gene expression, Glycosylations, Oxidations

Peer-Reviewed Papers
15

References

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