Insulin-transferrin-sodium selenite media supplement
γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture
DOWNLOAD MSDS (PDF)Synonym: ITS Supplement
-
Popular Documents: Product Information Sheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Cell Culture, Hybridoma Media Supplements, Hybridoma Platform, ITS and Other Supplements, Reagents and Supplements, |
| sterility | γ-irradiated |
| product line | BioXtra |
| form | lyophilized powder |
| suitability | suitable for cell culture |
| storage temp. | −20°C |
Description
Frequently Asked Questions
Live Chat and Frequently Asked Questions
are available for this Product.
Other Notes
Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
Quantity
Each vial sufficient to prepare 5 liters of medium.
Application
Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
Price and Availability
NEW! Sigma® Cultureware
Introducing Sigma’s new competitively priced line of cultureware:
- . Multiwell Plates
- . Culture Flasks
- . Cryogenic Vials & More
Customers Also Viewed
powder, BioReagent, suitable for cell culture
liquid, sterile-filtered, BioReagent, suitable for cell culture
liquid, sterile-filtered, BioReagent, suitable for cell culture
liquid, sterile-filtered, BioReagent, suitable for cell culture
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
