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I1891 Sigma

Pyrophosphatase, Inorganic from baker's yeast (S. cerevisiae)

≥90% (HPLC), lyophilized powder, ≥1,000 units/mg protein (BCA)

Synonym: Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

Description

Preparation Note

HPLC purified; Prepared from I1643

Unit Definition

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.

Physical form

lyophilized powder containing 90% buffer salts

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.

General description

Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.

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